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The principle of magnetic bead method for extracting nucleic acid
2023-07-26
The principle of extracting nucleic acid by magnetic bead method is based on the same principle as that of silica gel membrane centrifuge column. After the surface of Superparamagnetism nanoparticles is improved and modified by nanotechnology, Superparamagnetism silica nanobeads are prepared.Magnetic beads can specific……
Preparation of Blood Samples in RNA Research
2023-07-26
Reagents to be prepared: TRIzol (mainly phenol), chloroform (phenol: chloroform: Isoamyl alcohol=25:24:1), isopropanol, 80% alcohol (prepared with RNase free ddH2O), RNase free ddH2O.Experimental process1、 Sample Pretreatment① 1 milliliter of whole blood+1 milliliter of RNase free ddH2O.② Centrifuge RNA blood preservat……
Precautions for collecting body fluids for exosomes extraction
2023-07-26
Extracellular vesicles, also known as extracellular vesicles (EVs), are nanoscale vesicles actively secreted by all cells. Living cells release different types of extracellular vesicles into the extracellular environment for intercellular communication, and extracellular vesicles are increasingly considered promising b……
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