Model: CRNA-300
Carrier RNA is a mixture of RNA fragments ranging from 200 to 3000 nt, dissolved in RNA protectant (Simgen product number: 4003230) at a concentration of 6 μg/ μl.
Product Introduction
In the process of column purification of trace nucleic acids, such as virus RNA purification, virus DNA purification, trace DNA extraction and other experiments, the binding and elution efficiency of trace nucleic acids on the purification column is reduced, leading to insufficient recovery of PCR templates, which ultimately leads to detection failure. Adding Carrier RNA to the column purified nucleic acid purification system can improve the recovery efficiency of trace nucleic acid by more than 10 times. At the same time, because of the presence of Carrier RNA, it can also protect trace RNA templates and reduce the attack probability of RNA enzyme against trace RNA templates. Carrier RNA is an essential component in the purification of trace amounts of RNA, such as viral RNA.
Usage method
Add 1-5 to the lysis solution of each purification system for micro nucleic acid purification μ Carrier RNA. The final trace nucleic acid containing Carrier RNA can be directly used for PCR or RT-PCR reactions.