This magnetic bead is designed specifically for nucleic acid extraction and purification, with a large number of silane alcohol groups (hydroxyl groups) modified on the surface. It can specifically bind to nucleic acids in solution through hydrophobic, hydrogen bonding, and electrostatic interactions under high salt and low pH conditions, without binding to other impurities (such as egg white). It can quickly separate nucleic acids from biological samples, with a safe and simple operation, which is very conducive to the automation and high-throughput extraction of nucleic acids.
    【 Main parameters 】

	Beads OHseries	Beads OH1series	Beads OH2series
magnetism        nucleus	Fe3O4
shell        layer	Silicon oxide
Magnetic type	Superparamagnetism
Average particle size（Nm）	eighty	two hundred	five hundred
strong        Degree（Mg/mL）	eighty-seven point five	ninety	ninety

   
  Storage conditions and validity period
Storage: Room temperature for 6 months, 2-8 ℃ for 18 months.
  Features
1. Superparamagnetism and high magnetic responsiveness: magnetic beads prepared by special process have extremely fast reaction speed under the action of magnetic field, complete adsorption in a few seconds, and greatly shorten the extraction time.
2. Good suspension performance, good surface hydrophilicity of magnetic beads, good suspension performance in aqueous phase and various buffer solutions, and more efficient binding of nucleic acid and magnetic beads.
3. Good stress resistance, this magnetic bead overcomes the disadvantage of traditional agarose magnetic beads that are prone to expansion and rupture, and has good stability within the range of -20 ℃ to 60 ℃, meeting some extreme handling needs of customers. Good physical and chemical stability, with a complete silicon oxide layer, ensuring repeatability.
4. High yield: The magnetic beads have a unique surface structure of silicone alcohol, with higher adsorption capacity, making it easy to obtain a large amount of DNA/RNA.
5. High purity of nucleic acid products: The optimized buffer formula can remove various impurities in the extraction system, and the obtained nucleic acid can be directly used for downstream experiments, such as enzyme digestion and sequencing.
6. Magnetic beads have high compatibility and are suitable for use with various automation equipment to achieve high flux operation.
  Application direction
Purification of PCR products; Plasmid extraction; Virus nucleic acid extraction, genome extraction from samples such as blood, tissue, plants, and microorganisms.
  Magnetic bead performance
1. Nucleic acid binding capacity: more than 20 µ g/mg magnetic beads.
2. Using our company's whole blood magnetic bead extraction kit to extract 200 µ L of fresh human whole blood (approximately 5e6 white blood cells/mL) can generally obtain 6 µ g of genomic DNA, with OD260/OD280 values ranging from 1.7 to 1.9.
3. Extraction performance of free nucleic acid in plasma: The recovery rate of 100bp~1000bp nucleic acid fragments is 80%~90% (the shorter the fragment, the higher the recovery rate).
    

Fragment length	fluorescencePCRamplificationCtvalue
	Compare manufacturers' nucleic acid extraction kits	Our company's nucleic acid extraction kit
		Beads OHseries	Beads OH1series	Beads OH2series
112bp	nine point one nine	nine point two six	eight point six seven	eight point eight five
200bp	sixteen point one eight	sixteen point two three	fifteen point seven four	fifteen point four six
1000bp	thirteen point eight nine	fourteen point zero eight	thirteen point six five	thirteen point eight three

Note: The extraction results of adding three different lengths of nucleic acid fragments with known concentrations to plasma (compared with a well-known foreign manufacturer's nucleic acid extraction kit)
    
  【 Precautions 】
Before using this product, please make sure to oscillate or sonicate the magnetic beads thoroughly to maintain a uniform suspension state.
2. Operations such as freezing, drying, and centrifugation can cause agglomeration of magnetic beads, making them less prone to resuspension and dispersion, and affecting the chemical activity of functional groups on the surface of magnetic beads.
3. This product needs to be used in conjunction with magnetic separation equipment.
4. The extracted sample is saliva or anticoagulant whole blood: 100 is recommended μ L sample (200 μ L-300 μ L) Cracking liquid 20 μ L (20mg/mL) Proteinase K, 60 degrees reaction for 10 minutes, under this system, 50% μ L-300 μ Compare the extraction effect with isopropanol.
  Attention: It is best to use it before alcohol washing five hundred μ L Wash once with guanidine salt solution.
5. Coagulate blood, with a volume of 50 μ L. Others can refer to anticoagulant whole blood.
  Attention: It is best to use it before alcohol washing five hundred μ L Wash once with guanidine salt solution.
6. Oral swab, one swab uses 400 μ About 20 L of cracking liquid μ L (20mg/ml) Proteinase K, 60 degrees reaction for 10 minutes, add 400 μ Isopropanol of L.